Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing

Gonca Ozten Dere; Cagman Tan; Sezcan Mumusoglu; Pinar Zarakolu; Gurkan Bozdag

doi:10.21613/GORM.2023.1452

Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing

Authors

Gonca Ozten Dere tobb etu university hospital https://orcid.org/0000-0001-8951-3037
Cagman Tan Erciyes University https://orcid.org/0000-0001-6972-1349
Sezcan Mumusoglu Hacettepe University https://orcid.org/0000-0001-7000-7580
Pinar Zarakolu Hacettepe University https://orcid.org/0000-0003-4918-4085
Gurkan Bozdag Bahçeçi Health Group https://orcid.org/0000-0002-6679-9623

DOI:

https://doi.org/10.21613/GORM.2023.1452

Keywords:

Endometrial microbiota, Microbiota, Unexplained infertilit

Abstract

OBJECTIVE: The primary objective of our study was to compare the endometrial microbiota profiles of women with unexplained infertility and fertile women.

STUDY DESIGN: In this case control study a total of 15 patients with unexplained infertility and 15 patients who had a live birth in the last two years with proven fertility were recruited. Endometrial samples were collected and analyzed through next-generation sequencing of the bacteria-specific 16S ribosome gene.

RESULTS: Lactobacillus species represented the majority of the microbiome profile in both groups. The median percentage of the endometrial Lactobacillus between infertile patients and fertile patients was not statistically significant (p=0.9). Groups were divided into two categories: Lactobasillus dominant (Lactobacillus spp.> 90%, LD) and Non-Lactobacillus dominant (NLD). Lactobacillus dominance was detected in six of nine patients (66.7%) in the study group and four of nine patients (44.5%) in the control group, and no significant difference was observed between them (p=0.64).

CONCLUSION: According to our study results, no significant difference was observed between the endometrial microbiota profile of infertile and fertile patients. Further studies with larger sample sizes are needed to characterize the endometrial microbiota and its impact on reproduction.
Keywords: Microbiota, unexplained infertility, endometrial microbiota

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Published

2023-12-26

How to Cite

Ozten Dere G, Tan C, Mumusoglu S, Zarakolu P, Bozdag G. Comparison of Endometrial Microbiota Results Between Patients with Unexplained Infertility and Fertile Patients through 16s rRNA Sequencing. Gynecol Obstet Reprod Med [Internet]. 2023Dec.26 [cited 2024Dec.27];29(3):181-7. Available from: https://gorm.com.tr/index.php/GORM/article/view/1452

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Issue

Vol. 29 No. 3 (2023):

Section

Reproductive Medicine: Endocrinology and Infertility

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