Oxidative Stress in Endometrial Flushing Fluid of Patients with Polycystic Ovary Syndrome, Endometrioma and Uterine Leiomyoma: Comparison with Healthy Controls

Mustafa Demir; Onur Ince; Bulent Yilmaz; Mert Ulas Barut; Ulviye Cansu Ozturk; Avni Kılıc; Ahmet Berkiz Turp; Recep Sutcu; Seyithan Taysi

doi:10.21613/GORM.2020.1091

Authors

Mustafa Demir Anka hospital / Gaziantep https://orcid.org/0000-0002-0130-0254
Onur Ince Kutahya Health Science University Faculty of Medicine, Kutahya, Turkey https://orcid.org/0000-0003-2263-8956
Bulent Yilmaz Recep Tayyip Erdogan University Faculty of Medicine, Rize, Turkey http://orcid.org/0000-0002-9458-2253
Mert Ulas Barut Harran University Faculty of Medicine, Sanliurfa, Turkey http://orcid.org/0000-0002-7008-8218
Ulviye Cansu Ozturk Recep Tayyip Erdogan University Faculty of Medicine, Rize, Turkey https://orcid.org/0000-0002-1718-5422
Avni Kılıc Sanliurfa Education and Research Hospital, Sanliurfa, Turkey http://orcid.org/0000-0002-1223-9671
Ahmet Berkiz Turp Silifke State Hospital, Mersin, Turkey http://orcid.org/0000-0003-2981-1096
Recep Sutcu Izmir Katip Celebi University Faculty of Medicine, Izmir,Turkey http://orcid.org/0000-0002-6543-1130
Seyithan Taysi Gaziantep University, Faculty of Medicine, Gaziantep, Turkey https://orcid.org/0000-0003-1251-3148

DOI:

https://doi.org/10.21613/GORM.2020.1091

Keywords:

Endometrial flushing fluid oxidative stress, Endometrial receptivity, Endometrioma, Myoma uteri, Polycystic ovary syndrome

Abstract

OBJECTIVE: Polycystic ovary syndrome, myoma uteri and endometrioma are frequently seen gynecologic problems and all three diseases may cause infertility. Aim of this cross-sectional study was to compare total antioxidant status, ceruloplasmin, total sulfhydryl , total oxidant status, lipid hydroperoxide and oxidative stress index levels in endometrial flushing fluid of patients with Polycystic ovary syndrome (n=20), uterine leiomyoma (n=20), endometrioma (n=19), and healthy women (n=20).

STUDY DESIGN: We compare endometrial flushing fluid of patients with polycystic ovary syndrome (n=20), uterine leiomyoma (n=20), endometrioma (n=19) and healthy women (n=20). Endometrial flushing fluid samples were collected during the implantation window of all women.

RESULTS: Mean age of groups was 28.90±5.45, 37.25±2.73, 32.84±6.62 and 32.15±5.18 in Polycystic ovary syndrome, myoma uteri, endometrioma and control groups, respectively (p<0.05). Mean total antioxidant status, ceruloplasmin and total sulfhydryl levels indicating antioxidant state were comparable between Polycystic ovary syndrome, myoma uteri, endometrioma and control groups (p=0.806, p=0.156, p=0.328 respectively for markers). Similarly, oxidant state-related markers didn’t differ significantly between 4 groups (p=0.090 for total oxidant status, p=0.087 for lipid hydroperoxide, p=0.312 for oxidative stress index).

CONCLUSION: Endometrial flushing fluid total antioxidant status, total oxidant status, lipid hydroperoxide, ceruloplasmin, and total sulfhydryl levels during implantation window didn’t differ between women with Polycystic ovary syndrome, uterine leiomyoma, endometrioma, and healthy controls.