Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?

Tulay Irez; Sinem Ercan Dogan; Enver Ciraci; Saadet Busra Aksoyer; Muhammet Sait Toprak; Nurten Dayioglu; Onur Guralp

doi:10.21613/GORM.2020.1087

Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?

Authors

Tulay Irez Istanbul Yeni Yuzyil University Faculty of Medicine,Dept.of Histology&Embryology http://orcid.org/0000-0001-8272-4931
Sinem Ercan Dogan Istanbul Yeni Yuzyil University ,Histology and Embryology, Clinical Embryology Master program,Istanbul http://orcid.org/0000-0001-7904-4556
Enver Ciraci Biruni University ,Health Science Faculty, Istanbul http://orcid.org/0000-0001-5726-4734
Saadet Busra Aksoyer http://orcid.org/0000-0002-8338-9546
Muhammet Sait Toprak Biruni University Health Science Faculty,Istanbul http://orcid.org/0000-0002-5102-6599
Nurten Dayioglu http://orcid.org/0000-0003-4103-6045
Onur Guralp http://orcid.org/0000-0002-3517-3046

DOI:

https://doi.org/10.21613/GORM.2020.1087

Keywords:

Apoptosis, Cumulus cells, In vitro oocyte maturation human, Tunel assay

Abstract

OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes.

STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells.

RESULTS: In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p> 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).

CONCLUSION: In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter.

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Published

2021-12-15

How to Cite

Irez T, Ercan Dogan S, Ciraci E, Aksoyer SB, Toprak MS, Dayioglu N, Guralp O. Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?. Gynecol Obstet Reprod Med [Internet]. 2021Dec.15 [cited 2024Dec.22];27(3):234-41. Available from: https://gorm.com.tr/index.php/GORM/article/view/1087

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Issue

Vol. 27 No. 3 (2021)

Section

Reproductive Medicine: Endocrinology and Infertility

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